How Dolly the sheep was cloned

The scientists who produced Dolly used a technique that transferred the nucleus from a cell of an adult sheep (the donor cell) into an egg cell (the recipient cell). They obtained donor cells from the udder of an adult sheep and recipient cells from other sheep.

Before the nucleus was transferred from the donor cell to the recipient cell, the scientists used a high-powered microscope and a very fine micropipette to suck out the recipient cell's nucleus. (Inside the nucleus are chromosomes, the packages that contain the cell's DNA.)

Each donor cell was then forced into a state of 'quiescence' - where the DNA stops dividing – and placed alongside a recipient cell. The two cells were then encouraged to fuse by way of an electric pulse. The recipient egg cell now had 'new' DNA – that of the donor cell – with which to begin the process of cell division and growth. It was implanted in the uterus of yet another sheep and its progress monitored. Of the 277 original donor cells, only 29 made it to the stage of being implanted, and of those only one – Dolly – went full term.

In 1998, several laboratories announced the successful cloning of other species, although the rate of success remained low in all cases. A University of Hawaii lab has produced dozens of cloned mice, using a variation of the nuclear replacement technique used with Dolly. Instead of fusing the donor and recipient cells, the researchers inserted the nucleus of the donar cell directly into the recipient cell. The cells from which the donor nucleus was extracted are naturally quiescent cells found in the ovary.

According to Ian Wilmut, the leader of the team that produced Dolly, getting donor cells (or donor DNA) into a quiescent state is essential because it allows the reprogramming of the adult DNA. Nevertheless, scientists in a US lab claim they have produced cloned calves without establishing quiescence beforehand.

Performed by: Jorge Borbinha
Posted by: Rui Cóias

not have children in order to help the environment

Sarah and her fiancé have decided not to have children in order to help the environment.

Most young girls dream of marriage and babies. But Sarah dreamed of helping the environment and as she agonized over the perils of climate change, the loss of animal species, the destruction of wilderness and overpopulation, she came to the extraordinary decision never to have a child.
“I realized then that a baby would contribute to the pollution levels on the planet, and that never having a child was the most environmentally friendly thing I could do.
To my relief, Mark, my fiancé, agreed that having children is not a sensible decision nowadays. Overpopulation and the unbalanced distribution of people on Earth contribute to the loss of natural resources”.

Isabel Luz
posted by: Isabel Luz

Clonning of DNA - Biology

Paulo talhinhas
posted by: Isabel Luz

Bones

One of my favourite series is Bones, featuring Emily Deschanel and David Boreanaz, one of my favourite actors.

From executive producers Hart Hanson, Barry Josephson and Stephen Nathan the genially produced series BONES, a darkly amusing procedural with humor, heart and character, inspired by real-life forensic anthropologist and novelist Kathy Reichs.

Bones is a crime show on FOX. Emily Deschanel plays the title character Dr. Temperance "Bones" Brennan, and David Boreanaz plays Special Agent Seeley Booth. Together the two of them solve crimes that anyone else couldn´t solve and secretly love each other.

My favourite moment in the TV show was when Billy Gibbons(ZZ Top's vocals, guitar and keyboards)- in the series he is Angela´s father - visited Angela for a couple of days. Hojins went to talk to him to ask for Angela´s hand in marriege. Billy Gibbons stands for his habits and never takes of his sunglasses.

The series also features Jeffersonian's scientists: Angela Montenegro (Michaela Conlin), an artist who specializes in reconstructing facial features and crime scenes; "the bug guy", conspiracy theorist DR. Jack Hodjins (TJ Thyne), is an expert on insects, spores and minerals; and DR. Camille "CAM" Saroyan (Tamara Taylor), the street-smart and centered coroner. Lending his professional expertise to Brennan and Booth is DR. Lance Sweets (John Francis Daley), a brilliant young psychologist who is equally adept at profiling murderers and interrogating suspects.

Performed by: Jorge Borbinha
Posted by: Rui Cóias

Erin Brockovich

With no money, no job and without earning and money from the state because of accident when she was not guilty, Erin Brockovich asked to her lawyer, Ed Masry, a job in his office.
There Erin finds same documents about o problem with uranium in same places around of a factory.
Erin asked to the layer if she can investigate about the problem and he said yes.
Ed and Erin fight against the people who had polluted the places. That pollution had caused several health problems to the people o lived in the critical Zone.
Finally they won 333 million dollars given by the state.

written by: Ângela Martins
posted by: Isabel Brito da Luz

kareoke day

posted by: Isabel Luz

Casio CT-680 Review

Casio CT-680 is my current keyboard. This full-size stereo keyboard is one of the TONE BANK variety, meaning that it stores more than the standard 127 MIDI complement of sounds, it stores 220!
It is not touch sensitive but the overall sound is pretty crystal and has some varieties among the usual draft of pianos, brasses, guitars, basses, woodwind, strings and percussion. This Casio also has the ability to memorize one complete recording.
The keyboard utilization is pretty basic. I'd say it's pretty self explanatory without the manual but you should be prepared for some exploring. The number pads are easy to use but you have to get used to the way they work.
About the features, CT-680 has no sequencer and no expansion, but it does have one memory space but the song is only saved until the unit is unplugged. I only take it on the road sometimes. It does have MIDI in and out so that's helpful. And it also has an assignable jack, headphone jack, and a pedal jack. All of the mentioned before come in very handy. It does have a good amount of sound effects. I particulary like Strings, Elec Piano and Acous Guitar. It's not very fitting for the studio but it is suits me.

The keyboard can be split at the lower half, as is usual on Casio keyboard by selection of one of the two modes for chord accompaniment. Once selected the various parts of the backing can be switched on or off as desired - only the bass has not got a switch to remove it from the accompaniment.

There is a video is Youtube in wich the music “A Whither Shade of Pale” is played in E. The link is http://www.youtube.com/watch?v=fblX9o6WSh8. I know how to play it in A.

Performed by: Jorge Borbinha

Posted by: Rui Cóias

Halloween cartoon´s

ON HALLOWEEN

The witches fly
Across the sky,
The owls go, "Who? Who? Who?"
The black cats yowl
And green ghosts howl,
"Scary Halloween to you!"

A BOO-GRAM GREETING!

Green cats eyes
in midnight gloom
fly with the witch
on her ragged broom
Ghosts and shadow people
evade our sight.
None may not see what they might.
We begin our door to door Halloween flight.
Not knowing our immediate blight
we bravely approach your door and RING……
TRICK OR TREAT!! We say with a strong and mighty grin.
Candy and little toys we hope to gain
from this daring plan imagined in these little brains.
BOOOOO!! We are greeted as a scary hand
extends to our goodie sacks.
The lights go black, we stagger back!!
Screams explode, others jump!!
Dogs howl and unfriendly things give us a bump.
It’s Halloween! The voice screams as
we dump out our goodie sacks and run.

So keep the goodies and
BOOOOOOOOO! To you!

Isabel Brito da Luz

posted by:
Isabel Brito da Luz

Angela Martins

posted by:
Isabel Brito da Luz

Episode 5: Global Warming, It's All About Carbon

Angela Martins

posted by:
Isabel Brito da Luz

An Inconvenient Truth

Angela Martins

posted by:
Isabel Brito da Luz

Melissa Etheridge - I Need to Wake up

Angela Martins

posted by:
Isabel Brito da Luz

Albert Arnold “Al” Gore Jr.

Al Gore was born in Washington, on the 31st March of 1948, politician (Democratic Party) and environmental activist. It was Vice President of USA since 1993 to 2000. He last the Presidential elections, in 2000, against George W. Bush.
In 1993 he wrote “Earth in the Balance: Ecology and the Human Spirit” and in 2006 “An Inconvenient truth”) about global warming which received the best documentary award in 2007.
In 2007, Al Gore received the Nobel with the Intergovernamental Panel on Climate Change from the United Nations, “For their efforts to build up and disseminate greater knowledge about man-made climate change, and to lay the foundations for the measures that are needed to counteract such change”.
In 2007 he received, the Prince of Astories de la concondia prize (award) in Oviedo, giver by prince of Astories foundation.

written by:
Ana Cachucho
Isabel Brito da Luz

posted by:
Isabel Brito da Lua